ToRCH IgG/IgM Combo Rapid Test Cassette

A rapid test for the qualitative detection of IgG antibodies to Toxoplasma gondii (Toxo), Rubella virus (Rubella), Cytomegalovirus (CMV) and Herpes simplex virus 1/2 (HSV 1/2) in human whole blood, serum or plasma

1 box has 25 tests

For Export Only

ToRCH IgG Combo Rapid Test Cassette is a qualitative, lateral flow immunoassay for the detection of IgG antibodies to Toxo, Rubella, CMV, and HSV 1/2 in whole blood, serum or plasma specimens.

Toxo IgG Rapid Test/ Rubella IgG Rapid Test/CMV IgG Rapid Test:

In this test, anti-human IgG are coated in the test line regions of each section in the test. During testing, the whole blood, serum or plasma specimen reacts with T.gondii antigen, Rubella antigen, CMV antigen coated particles in the test strip. The mixture then migrates upward on the membrane by capillary action and reacts with the anti-human IgG on the membrane in the test line region respectively. The presence of a colored line in the test line region indicates a positive result for T.gondii, Rubella, CMV infection, while its absence indicates a negative result for that infection.

HSV 1/2 IgG Rapid Test:

In this test, antigens of HSV 1/2 are coated in the test line region of the test. During testing, the whole blood, serum or plasma specimen reacts with anti-human IgG coated particles in the test strip. The mixture then migrates upward on the membrane by capillary action and reacts with the HSV 1/2 specific antigens on the membrane in the test line region. The presence of a colored line in the test line region indicates a positive result for HSV 1/2 IgG, while its absence indicates a negative result.

To serve as a procedural control, a colored line will always appear in the respective control line regions of all the four strips indicating that proper volume of specimen has been added and membrane wicking has occurred.

Materials Provided 

• Test Cassettes
• Droppers
• Buffer
• Package Insert

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

ToRCH IgG Combo Rapid Test

1. Remove the test cassette from the sealed pouch and use it within one hour. Best results will be obtained if the assay is performed as soon as possible.
2. Place the test cassette on a clean and level surface. Hold the dropper vertically; draw the specimen about 1 cm above the upper end of the nozzle as shown in illustration below. Transfer 1 full drop (approx. 20μL) of specimen to each specimen well, then add 2 drops of buffer (approx. 80μL) to each specimen well and start the timer. See the illustration below.
3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret results after 20 minutes.

Interpretation of results

Toxo Positive: *Two colored lines appear in the “Toxo” section. One line should be in the control line region (C) and another line should be in the test line region (T).

Rubella Positive: *Two colored lines appear in the “Rub” section. One line should be in the control line region (C) and another line should be in the test line region (T).

CMV Positive: *Two colored lines appear in the “CMV” section. One line should be in the control line region (C) and another line should be in the test line region (T).

HSV 1/2 Positive: *Two colored lines appear in the “HSV 1/2” section. One line should be in the control line region (C) and another line should be in the test line region (T).

*Note: The intensity of the color in test line region (T) will vary depending on the concentration of IgG and/or IgM antibodies present in the specimen. Therefore, any shade of color in test line region (T) should be considered positive.

NEGATIVE: One colored line appears in the control line region (C) of every section. Non-appearance of a visible line in the test line region (T) of any section is indicative of a negative test result for that specific section, viz. Toxo, Rub, CMV, and HSV 1/2.

INVALID: The control line fails to appear in any section. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.